Complete 5 pages APA formatted article: High Performance Liquid Chromatography.


Complete 5 pages APA formatted article: High Performance Liquid Chromatography. The retention time is the amount of time it takes for the analyte to elute and is unique in each sample. The linear velocity that is enhanced by the high pressure that is used gives the components minimal time to diffuse inside the column. This is necessary because it allows for a higher resolution of the chromatogram that is used to deduce results. In a typical HPLC, various miscible organic liquids are used and the modern units have the capacity to generate a maximum pressure of 6000psi. HPLC units are available commercially and the buyer selects a type that is most suitable for their need. Different types come with different detectors, for example, UV absorption detector, refractive index detector, conductivity detector, evaporative light scattering detector, fluorescence detector, and electrochemical detector.Generally, there are two types of HPLC units, one being the preparative HPLC and the other an analytical HPLC. In the preparative type, compounds are usually isolated and purified whereas the analytical mainly gets detailed information about a sample that is passed through it. This means that the sample can be identified, quantified and its resolution obtained. The main uses of HPLC, however, are identification and purification of samples. HPLC is highly preferred by many professionals because it is accurate, sensitive, simple to use and can analyze nonvolatile samples that are sensitive to temperature changes. Furthermore, HPLC can easily be used in combination with mass spectrophotometry as a means of validating the data collected. Most chemists use the technique in the analysis of amino acids, hydrocarbon, proteins, pesticides, pharmaceuticals, among other products.A combination of 0.7mg/ml phenol, 4.0mg/ml toluene and 5.0mg/ml ethylbenzene is dissolved in 10ml isopropanol. The amount of each of the above components is then calculated accordingly to allow for later calculations.

Don't use plagiarized sources. Get Your Custom Essay on
Complete 5 pages APA formatted article: High Performance Liquid Chromatography.
Just from $10/Page
Order Essay

Calculate the price of your order

550 words
We'll send you the first draft for approval by September 11, 2018 at 10:52 AM
Total price:
The price is based on these factors:
Academic level
Number of pages
Basic features
  • Free title page and bibliography
  • Unlimited revisions
  • Plagiarism-free guarantee
  • Money-back guarantee
  • 24/7 support
On-demand options
  • Writer’s samples
  • Part-by-part delivery
  • Overnight delivery
  • Copies of used sources
  • Expert Proofreading
Paper format
  • 275 words per page
  • 12 pt Arial/Times New Roman
  • Double line spacing
  • Any citation style (APA, MLA, Chicago/Turabian, Harvard)

Our guarantees

Delivering a high-quality product at a reasonable price is not enough anymore.
That’s why we have developed 5 beneficial guarantees that will make your experience with our service enjoyable, easy, and safe.

Money-back guarantee

You have to be 100% sure of the quality of your product to give a money-back guarantee. This describes us perfectly. Make sure that this guarantee is totally transparent.

Read more

Zero-plagiarism guarantee

Each paper is composed from scratch, according to your instructions. It is then checked by our plagiarism-detection software. There is no gap where plagiarism could squeeze in.

Read more

Free-revision policy

Thanks to our free revisions, there is no way for you to be unsatisfied. We will work on your paper until you are completely happy with the result.

Read more

Privacy policy

Your email is safe, as we store it according to international data protection rules. Your bank details are secure, as we use only reliable payment systems.

Read more

Fair-cooperation guarantee

By sending us your money, you buy the service we provide. Check out our terms and conditions if you prefer business talks to be laid out in official language.

Read more

Order your essay today and save 30% with the discount code ESSAYSHELP